| 质粒名称: | pAM-PAT-ProUBQ10 GW |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 79751 |
| 存储实验室: | Nico Dissmeyer, Arp Schnittger |
|
载体骨架基本信息 |
|
| 载体名称: | Binary vector pAMPAT-MCS |
| 完整质粒大小: | 8320bp |
| 载体类型: | 植物表达 |
| 筛选标记： | Basta |
| 载体抗性: | Chloramphenicol and Ampicillin |
| 生长温度: | 37 ℃ |
| 宿主菌: | ccdB Survival |
| 拷贝数: | 低拷贝 |
|
插入基因信息 |
|
| 启动子: | ProUBQ10 |
| 克隆方法: | Gateway Cloning |
| 5’测序引物 | gaccggcaacaggattcaatcttaag |
| 3’测序引物 | GAATTAGAAATTTTATTGATAGAAG |
|
文献引用方法 |
|
| 材料和方法部分: |
pAM-PAT-ProUBQ10 GW was a gift from Nico Dissmeyer & Arp Schnittger (Addgene plasmid # 79751 ; http://n2t.net/addgene:79751 ; RRID:Addgene_79751)
|
| 参考文献部分: | Phenotypes on demand via switchable target protein degradation in multicellular organisms. Faden F, Ramezani T, Mielke S, Almudi I, Nairz K, Froehlich MS, Hockendorff J, Brandt W, Hoehenwarter W, Dohmen RJ, Schnittger A, Dissmeyer N. Nat Commun. 2016 Jul 22;7:12202. doi: 10.1038/ncomms12202. 10.1038/ncomms12202 PubMed 27447739 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pAM-PAT-ProUBQ10 GW 8320 bp ds-DNA circular SYN 13-MAY-2021
DEFINITION binary DEST vector for plant expression.
ACCESSION .
VERSION .
KEYWORDS pAM-PAT-ProUBQ10 GW
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8320)
AUTHORS Faden F, Ramezani T, Mielke S, Almudi I, Nairz K, Froehlich MS,
Hockendorff J, Brandt W, Hoehenwarter W, Dohmen RJ, Schnittger A,
Dissmeyer N
TITLE Phenotypes on demand via switchable target protein degradation in
multicellular organisms.
JOURNAL Nat Commun. 2016 Jul 22;7:12202. doi: 10.1038/ncomms12202.
PUBMED 27447739
REFERENCE 2 (bases 1 to 8320)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..8320
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 348..372
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
rep_origin 484..1193
/label=oriV
/note="incP origin of replication"
promoter 1266..1357
/gene="bla"
/label=AmpR promoter
CDS 1358..2218
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(1576..1595)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 2389..2977
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 2878..2897
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
misc_feature 3289..3313
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
terminator 3407..3659
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
CDS complement(3679..4230)
/codon_start=1
/gene="Streptomyces hygroscopicus bar"
/product="phosphinothricin acetyltransferase"
/label=BlpR
/note="confers resistance to bialophos or phosphinothricin"
/translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
WQLDFSLPVPPRPVLPVTEI"
promoter complement(4272..4451)
/label=NOS promoter
/note="nopaline synthase promoter"
protein_bind 6593..6717
/gene="mutant version of attR"
/label=attR1
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
promoter 6742..6772
/label=lac UV5 promoter
/note="E. coli lac promoter with an ""up"" mutation"
CDS 6826..7485
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/label=CmR
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
primer_bind complement(6892..6911)
/label=CAT-R
/note="Chloramphenicol resistance gene, reverse primer"
CDS 7827..8132
/codon_start=1
/gene="ccdB"
/product="CcdB, a bacterial toxin that poisons DNA gyrase"
/label=ccdB
/note="Plasmids containing the ccdB gene cannot be
propagated in standard E. coli strains."
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
primer_bind 8011..8030
/label=ccdB-fwd
/note="ccdB gene, forward primer"
protein_bind complement(8173..8297)
/gene="mutant version of attR"
/label=attR2
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
ORIGIN
1 agcttgttga gaattcatcg cccgggactg ggttaacttc cgatcgattc tagactagtt
61 ctagagtccg caaaaatcac cagtctctct ctacaaatct atctctctct atttttctcc
121 agaataatgt gtgagtagtt cccagataag ggaattaggg ttcttatagg gtttcgctca
181 tgtgttgagc atataagaaa cccttagtat gtatttgtat ttgtaaaata cttctatcaa
241 taaaatttct aattcctaaa accaaaatcc agtgaccggg cggccgccac cgcggtggag
301 ggggatcaga ttgtcgtttc ccgccttcag tttaaactat cagtgtttga caggatatat
361 tggcgggtaa acctaagaga aaagagcgtt tattagaata atcggatatt taaaagggcg
421 tgaaaaggtt tatccgttcg tccatttgta tgtgtacatc accgacgagc aaggcaagac
481 cgagcgcctt tccgacgctc accgggctgg ttgccctcgc cgctgggctg gcggccgtct
541 atggccctgc aaacgcgcca gaaacgccgt cgaagccgtg tgcgagacac cggccgccgg
601 cgttgtggat acctcgcgga aaacttggcc ctcactgaca gatgaggggc ggacgttgac
661 acttgagggg ccgactcacc cggcgcggcg ttgacagatg aggggcaggc tcgatttcgg
721 ccggcgacgt ggagctggcc agcctcgcaa atcggcgaaa acgcctgatt ttacgcgagt
781 ttcccacaga tgatgtggac aagcctgggg ataagtgccc tgcggtattg acacttgagg
841 ggcgcgacta ctgacagatg aggggcgcga tccttgacac ttgaggggca gagtgctgac
901 agatgagggg cgcacctatt gacatttgag gggctgtcca caggcagaaa atccagcatt
961 tgcaagggtt tccgcccgtt tttcggccac cgctaacctg tcttttaacc tgcttttaaa
1021 ccaatattta taaaccttgt ttttaaccag ggctgcgccc tgtgcgcgtg accgcgcacg
1081 ccgaaggggg gtgccccccc ttctcgaacc ctcccggccc gctaacgcgg gcctcccatc
1141 cccccagggg ctgcgcccct cggccgcgaa cggcctcacc ccaaaaatgg cagcgctggc
1201 agtccttgcc attgccggga tcggggcagt aacgggatgg gcgatcagcc cgaaagctac
1261 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
1321 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
1381 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
1441 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
1501 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
1561 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
1621 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
1681 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
1741 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
1801 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
1861 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
1921 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
1981 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
2041 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
2101 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
2161 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
2221 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
2281 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
2341 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
2401 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
2461 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
2521 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
2581 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
2641 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
2701 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
2761 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
2821 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
2881 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
2941 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
3001 acggttcctg gccttttgct ggccttttgc tcacatggac tctagctaga ggatcacagg
3061 cagcaacgct ctgtcatcgt tacaatcaac atgctaccct ccgcgagatc atccgtgttt
3121 caaacccggc agcttagttg ccgttcttcc gaatagcatc ggtaacatga gcaaagtctg
3181 ccgccttaca acggctctcc cgctgacgcc gtcccggact gatgggctgc ctgtatcgag
3241 tggtgatttt gtgccgagct gccggtcggg gagctgttgg ctggctggtg gcaggatata
3301 ttgtggtgta aacaaattga cgcttagaca acttaataac acattgcgga cgtttttaat
3361 gatcgaatac taacgtctct accagatatc agcttgcatg ccggtcgatc tagtaacata
3421 gatgacaccg cgcgcgataa tttatcctag tttgcgcgct atattttgtt ttctatcgcg
3481 tattaaatgt ataattgcgg gactctaatc ataaaaaccc atctcataaa taacgtcatg
3541 cattacatgt taattattac atgcttaacg taattcaaca gaaattatat gataatcatc
3601 gcaagaccgg caacaggatt caatcttaag aaactttatt gccaaatgtt tgaacgatct
3661 gcttgactct aggggtcatc agatttcggt gacgggcagg accggacggg gcggcaccgg
3721 caggctgaag tccagctgcc agaaacccac gtcatgccag ttcccgtgct tgaagccggc
3781 cgcccgcagc atgccgcggg gggcatatcc gagcgcctcg tgcatgcgca cgctcgggtc
3841 gttgggcagc ccgatgacag cgaccacgct cttgaagccc tgtgcctcca gggacttcag
3901 caggtgggtg tagagcgtgg agcccagtcc cgtccgctgg tggcgggggg agacgtacac
3961 ggtcgactcg gccgtccagt cgtaggcgtt gcgtgccttc cagggacccg cgtaggcgat
4021 gccggcgacc tcgccgtcca cctcggcgac gagccaggga tagcgctccc gcagacggac
4081 gaggtcgtcc gtccactcct gcggttcctg cggctcggta cggaagttga ccgtgcttgt
4141 ctcgatgtag tggttgacga tggtgcagac cgccggcatg tccgcctcgg tggcacggcg
4201 gatgtcggcc gggcgtcgtt ctgggctcat ggtagatccc ctcgatcgag ttgagagtga
4261 atatgagact ctaattggat accgagggga atttatggaa cgtcagtgga gcatttttga
4321 caagaaatat ttgctagctg atagtgacct taggcgactt ttgaacgcgc aataatggtt
4381 tctgacgtat gtgcttagct cattaaactc cagaaacccg cggctcagtg gctccttcaa
4441 cgttgcggtt ctgtcagttc caaacgtaaa acggcttgtc ccgcgtcatc ggcgggggtc
4501 ataacgtgac tcccttaatt ctccgctcat gatcagattg tcgtttcccg ccttcggttt
4561 gggcgcgcca tttaaatagt ctagctcaac agagctttta acccaaattg gtacaataga
4621 atacaacttt agatcataat tctcaaaaga aagagattcc ttagctattc tatctgccac
4681 tccatttcct tctcggcttg tatgcacaag cataaaatcc tcaaacttgc taagtagata
4741 ctttatgtct tggataattg gattgagact tgacaagcat aactttcatg taaccaaaga
4801 cacaagttgc tgagaatcca cctcaaaaat gatcttccta taattgaatc gggataatga
4861 cagcacagcc catctaagag cctccacttc tacttccagc acgcttctta cttttaccac
4921 agctcttgca cctaaccata acaccttccc tgtatgatcg cgaagcaccc accctaagcc
4981 acattttaat ccttctgttg gccatgcccc atcaaagttg cacttaaccc aagattgtgg
5041 tggagcttcc catgtttctc gtctgtcccg acggtgttgt ggttggtgct ttccttacat
5101 tctgagcctc tttccttcta atccactcat ctgcatcttc ttgtgtcctt actaatacct
5161 cattggttcc aaattccctc cctttaagca ccagctcgtt tctgttcttc cacagcctcc
5221 caagtatcca agggactaaa gcctccacat tcttcagatc aggatattct tgtttaagat
5281 gttgaactct atggaggttt gtatgaactg atgatctagg accggataag ttcccttctt
5341 catagcgaac ttattcaaag aatgttttgt gtatcattct tgttacattg ttattaatga
5401 aaaaatatta ttggtcattg gactgaacac gagtgttaaa tatggaccag gccccaaata
5461 agatccattg atatatgaat taaataacaa gaataaatcg agtcaccaaa ccacttgcct
5521 tttttaacga gacttgttca ccaacttgat acaaaagtca ttatcctatg caaatcaata
5581 atcatacaaa aatatccaat aacactaaaa aattaaaaga aatggataat ttcacaatat
5641 gttatacgat aaagaagtta cttttccaag aaattcactg attttataag cccacttgca
5701 ttagataaat ggcaaaaaaa aacaaaaagg aaaagaaata aagcacgaag aattctagaa
5761 aatacgaaat acgcttcaat gcagtgggac ccacggttca attattgcca attttcagct
5821 ccaccgtata tttaaaaaat aaaacgataa tgctaaaaaa atataaatcg taacgatcgt
5881 taaatctcaa cggctggatc ttatgacgac cgttagaaat tgtggttgtc gacgagtcag
5941 taataaacgg cgtcaaagtg gttgcagccg gcacacacga gtcgtgttta tcaactcaaa
6001 gcacaaatac ttttcctcaa cctaaaaata aggcaattag ccaaaaacaa ctttgcgtgt
6061 aaacaacgct caatacacgt gtcattttat tattagctat tgcttcaccg ccttagcttt
6121 ctcgtgacct agtcgtcctc gtcttttctt cttcttcttc tataaaacaa tacccaaaga
6181 gctcttcttc ttcacaattc agatttcaat ttctcaaaat cttaaaaact ttctctcaat
6241 tctctctacc gtgatcaagg taaatttctg tgttccttat tctctcaaaa tcttcgattt
6301 tgttttcgtt cgatcccaat ttcgtatatg ttctttggtt tagattctgt taatcttaga
6361 tcgaagacga ttttctgggt ttgatcgtta gatatcatct taattctcga ttagggtttc
6421 atagatatca tccgatttgt tcaaataatt tgagttttgt cgaataatta ctcttcgatt
6481 tgtgatttct atctagatct ggtgttagtt tctagtttgt gcgatcgaat ttgtcgatta
6541 atctgagttt ttctgattaa cagcctcgag aaagaggatc cacctgagga tcacaagttt
6601 gtacaaaaaa gctgaacgag aaacgtaaaa tgatataaat atcaatatat taaattagat
6661 tttgcataaa aaacagacta cataatactg taaaacacaa catatccagt cactatggcg
6721 gccgcattag gcaccccagg ctttacactt tatgcttccg gctcgtataa tgtgtggatt
6781 ttgagttagg atccgtcgag attttcagga gctaaggaag ctaaaatgga gaaaaaaatc
6841 actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt
6901 cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta
6961 aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat tcttgcccgc
7021 ctgatgaatg ctcatccgga attccgtatg gcaatgaaag acggtgagct ggtgatatgg
7081 gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc
7141 tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca agatgtggcg
7201 tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat gtttttcgtc
7261 tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa tatggacaac
7321 ttcttcgccc ccgttttcac catgggcaaa tattatacgc aaggcgacaa ggtgctgatg
7381 ccgctggcga ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt
7441 aatgaattac aacagtactg cgatgagtgg cagggcgggg cgtaaacgcg tggatccggc
7501 ttactaaaag ccagataaca gtatgcgtat ttgcgcgctg atttttgcgg tataagaata
7561 tatactgata tgtatacccg aagtatgtca aaaagaggta tgctatgaag cagcgtatta
7621 cagtgacagt tgacagcgac agctatcagt tgctcaaggc atatatgatg tcaatatctc
7681 cggtctggta agcacaacca tgcagaatga agcccgtcgt ctgcgtgccg aacgctggaa
7741 agcggaaaat caggaaggga tggctgaggt cgcccggttt attgaaatga acggctcttt
7801 tgctgacgag aacaggggct ggtgaaatgc agtttaaggt ttacacctat aaaagagaga
7861 gccgttatcg tctgtttgtg gatgtacaga gtgatattat tgacacgccc gggcgacgga
7921 tggtgatccc cctggccagt gcacgtctgc tgtcagataa agtctcccgt gaactttacc
7981 cggtggtgca tatcggggat gaaagctggc gcatgatgac caccgatatg gccagtgtgc
8041 cggtctccgt tatcggggaa gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa
8101 acgccattaa cctgatgttc tggggaatat aaatgtcagg ctcccttata cacagccagt
8161 ctgcaggtcg accatagtga ctggatatgt tgtgttttac agtattatgt agtctgtttt
8221 ttatgcaaaa tctaatttaa tatattgata tttatatcat tttacgtttc tcgttcagct
8281 ttcttgtaca aagtggtgat cctccgttcc atgggctaga
//
载体图谱质粒图谱pdf版和相关资料下载
载体质粒应用举例
- 上一篇：pcDNA4-Beclin1-HA
- 下一篇：pET28a-UVRAG CC
